Molarity Calculator Dna. 7 ×10−18mol \pu 6. Calculate insert mass from vec

7 ×10−18mol \pu 6. Calculate insert mass from vector mass and molar ratios (3:1, 5:1) for T4 DNA ligase reactions. If your DNA has a known molar mass different from the default, adjust this value accordingly. RNA splicing modulator 6 causes accumulation of unspliced RNA in the Hs-Hsh155 strain. 18) + (G n x 329. Lab Calculators DNA Calculator Calculate basic chemical properties of a DNA or RNA molecule. I know the molecular weight and extinction coefficient of the oligos. 21) + (T n x 304. Calculate nucleic acid DNA Molarity Calculator Tool The DNA Molarity Calculator is a fast, precise, and educational tool designed for students, researchers, and professionals in genetics, molecular biology, and clinical labs. Here are some specific applications where you may use the dsDNA Mass Moles Converter: 1. If you choose a custom Apr 15, 2014 · These include four DNA calculators (ligation calculator and three unit conversion calculators for double-stranded DNA), two RNA calculators (mass-to-moles and moles-to-mass conversions), and three DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. This is presumably a helix initiation energy. This calculator is useful for diluting DNA samples. Conversely, you can calculate the molarity of a nucleic acid solution prepared by dissolving a certain amount of it in a specified volume of a solvent. This will lose some accuracy. 96 A n, T n, C n, and G n are the number of each respective nucleotide within the polynucleotide. To prepare a solution of specific molarity based on mass, please use the Mass Molarity Calculator. Jul 4, 2022 · Here you will learn: What is DNA concentration, and how do we calculate it; How to calculate the DNA concentration from A 260 values; The DNA concentration equation (both for nucleic acid samples and oligonucleotides). , desired volume and molarity). Insert: vector molar ratio should be 6:1 to promote multiple inserts. Calculate concentration from 260 nm absorbance. Jan 9, 2026 · Use VectorBuilder’s DNA calculator to retrieve molecular weight and amount of your DNA (mass or moles) for your experiment like PCR, vector cloning, and more. This calculator is useful for diluting primers and DNA oligos. The DNA Copy-Number ↔ Molarity Converter turns an error-prone calculation into a one-click service for everyone from undergraduates to GMP viral-vector facilities. Same Different Different, import values Different, import values and names I have been out of the lab for sometime but I need to do a sequencing run. The Mass Molarity Calculator is commonly used in molecular biology applications that involve calculating a solution's molarity based on the solute's mass and the solvent's volume. The following example outlines how to calculate the volumes of primers and probe needed such that the primers and probe will be at a desired final concentration in a 5’ nuclease reaction. Enter the molecular weight, desired molarity and desired final volume. Useful for genetic research, PCR preparation, and sequencing work. This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for nucleic acids, and vice versa. Calculate volumes and concentrations in seconds. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions. Select the polymerase or kit from the list of products. The OligoCalc DNA MW calculations assume that there is not a 5' monophosphate) Anhydrous Molecular Weight = (A n x 313. Enter vector & insert sizes and get the exact ng of insert DNA needed for a 3:1 (or custom) molar ratio—no spreadsheets, 100 % browser-side. ProtParam [Documentation / Reference] is a tool which allows the computation of various physical and chemical parameters for a given protein stored in UniProtKB or for a user entered protein sequence. RNA splicing modulator 6 (Compound c7) is a RNA splicing modulator. e. Essential for molecular biology and genetic research. The calculator is designed to accommodate different ligation scenarios to help optimize your experimental outcomes. DNA is the basic unit of life, made up of nucleotides. - Mechanism of Action & Protocol. Jun 25, 2024 · This tool helps you accurately dilute a DNA stock solution to achieve a specified DNA copy number per microliter (µL). Otherwise just use the default value. DNA quantification: Determining the DNA concentration in a sample is crucial for many experiments. Dilute solution to a desired Molarity. Mar 25, 2025 · You can use DNA Calculator to: Calculate basic physical and chemical parameters of a nucleic acid molecule. This tool will calculate the molarity of a solution for a compound of known molecular weight. The concentration of plasmid A and B is 580 ng/ul and 1265 ng/ul, respectively. I would imagine that ionic strength would play a big role in the detergent:DNA stoichiometry by reducing the affinity of the detergent-DNA interaction. DNA & RNA Molarity, Mass, and Molecule Count Converter - Chrome Extension This handy Chrome extension provides an easy-to-use DNA/RNA calculator designed for researchers and scientists working with nucleic acids. RNA splicing modulator 6 reduces proliferation of chronic myeloid leukemia cells harboring the cancer-associated SRSF2P95H mutation. This calculator is particularly useful when preparing solutions with precise concentrations of specific compounds. Tm Calculator Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. 21) - 61. Vector: Insert molar ratios between 1:1 and 1:10 are optimal for single insertions (up to 1:20 for short adaptors). Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. Dec 28, 2024 · Online DNA Calculator: Determine molar concentration, DNA quantity, and dilution requirements in seconds Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. 2. DNA/RNA Molarity Calculator PRE-CLINICAL RESEARCH SERVICES: Pharm/Tox Testing, IC50 for 100+ Cancer Cell Lines 80 Xenograft Models: Anti-Tumor Activity Test In Vivo Liposome Encapsulation Services: siRNA, mRNA, DNA ELISA and Cell-Based Assay Development GENERATION OF STABLE CELL LINES: Generation of Stable Cell Line in 28 Days Dec 28, 2024 · Online DNA Calculator: Determine molar concentration, DNA quantity, and dilution requirements in seconds Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. 7 × 10 18 m o l. DNA Molecular Weight (typically for synthesized DNA oligonucleotides. ChemDraw feature - Generate chemical structures from SMILES (Simplified Molecular Input Line Entry System). Spaces allowed. Note that an anealing 3 days ago · Use VectorBuilder’s DNA calculator to retrieve molecular weight and amount of your DNA (mass or moles) for your experiment like PCR, vector cloning, and more. Westlab's Nucleic Acid Concentration & Molar Converter provides a seamless way to switch between all common units used for quantifying DNA and RNA. 5ug). Dilute solution to a desired concentration (mass/vol). double strand DNA base weight is 650 Dalton, for single strand is 330, for RNA is 340 Dalton. Calculate the mass or volume required to prepare a nucleic acid solution of specified molar concentration. Jan 27, 2025 · Parts per million (ppm) and parts per billion (ppb) to molarity calculator, DNA Tm Calculator - quickly calculate the Tm of the DNA/PCR primer to help designing primers for PCR/RT-PCR amplification, also generate DNA reverse compliment. Use the DNA Molecular Weight Calculator to determine the molar mass of single- or double-stranded DNA sequences accurately and instantly. It is used for oligonucleotides synthesis. Calculate DNA/RNA concentration, purity ratio, and yield from spectrophotometer readings. Overview Convert between weight and molar concentration for nucleic acids. The calculator will determine the amount of compound to add to a solution to achieve the desired molarity. Molarity Calculator The calculators on this page are independent and can be used in any order. 2) + (C n x 289. DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. Easy DNA molarity calculator with formula, chart, and export options. RNA splicing modulator 6 induces changes in pre-mRNA splicing. Use the DNA concentration calculator to find the concentration of the RNA, DNA, or custom oligonucleotide sequence. How to calculate different DNA molarity ratios between 2 plasmids by keeping the total amount of DNA same? I have two plasmids: plasmid A (8434bp)and plasmid B (12,082bp). The calculators are numbered because sometimes the results of one calculator are used as inputs to a later one. Instructions Select the product group of the polymerase or kit you plan to use. Can the ligation calculator be used for different types of DNA ligations? Yes, the QIAGEN Ligation Calculator can be used for various types of DNA ligations, including sticky end ligations, blunt end ligations, and TA cloning. I have a qubit measurement of my DNA library at 25ng/ul and the average… DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. Amount of substance is \pu6. Calculate base-pair molarity of dsDNA solutions. Calculate molarity, mass or volume. , stock solution molarity and volume) and "2" represents the diluted conditions (i. Support for 2-15 DNA fragments with automatic molar ratio calculations. Note: This calculation maybe useful: → When you extract DNA or RNA, the sequence was known, and you already measured the concentration, then you can get the copy number of the DNA or RNA by this calculation. If the diameter of this spherical cell is 1 micrometer, what would be the molar concentration of DNA in this cell? The volume comes to be \pu2 ×10−5L \pu 2 × 10 5 L. Dive into the world of DNA concentration today! Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. NEB-compatible calculations with formulas. Finally, adding an adjustment for salt gives the equation that the Oligo Calculator uses: No adjustment constant for salt concentration is needed, since the various parameters were determined at 1 Molar NaCl, and the log of 1 is zero. Unylinker-OH Resin (Substitution:350±25 mmol/g) is a macroporous polystyrene resin functionalized with Unylinker active groups with uniform particle size and uniform active groups. It helps you convert DNA concentration into molarity (mol/L, µM, nM) and estimate the number of DNA molecules per microliter (molecules/µL). Free online tool for researchers—convert ng/µl to nM, measure purity (A260/A280), and more! This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for nucleic acids, and vice versa. I want to do co-transfection of these plasmids in different ratios of molarity by keeping the total DNA amount same (1. Looking to calculate your DNA molarity? Use our Sambrook Calculator for accurate and quick results! Explore our DNA Molarity Calculator for quick, accurate calculations. It gives you the tools and knowledge you need. Calculate nucleic acid This calculator could figure out the DNA molecular weight of one or multiple FASTA sequences simultaneously which are entered directly or uploaded with txt or fasta file extension. Choose the organism you DNA originates from, or choose "Custom DNA fragment". Use NEBioCalculator to calculate molar ratios. It's essential for molecular biology applications like PCR, sequencing, and cloning. The converter helps researchers calculate the amount of DNA in moles based on the mass of dsDNA. This guide will help you learn how to find the mass of DNA molecules. NEBuilder Protocol Calculator can generate a protocol for your NEBuilder HiFi reaction when given a set of DNA fragments. Gibson Assembly Calculator Calculate optimal fragment ratios and design overlaps for seamless Gibson assembly. Jan 19, 2014 · A bacterium has a single copy of a \pu4 ×106bp \pu 4 × 10 6 b p circular genomic DNA. We would like to show you a description here but the site won’t allow us. Test description Unit of Measure for Library Library Size (bp) The mass molarity calculator tool calculates the mass of compound required to achieve a specific molar concentration and volume. The calculator uses the formula M 1 V 1 = M 2 V 2 where "1" represents the concentrated conditions (i. Next, select the source of Sep 3, 2024 · DNA Molar Mass Calculator Calculate Knowing the molar mass of DNA is key for scientists in molecular biology, biochemistry, and genetics. DNA/RNA molarity Calculator: M = w / MW Where: M: DNA molarity, in mol w: DNA weight, in g MW: DNA molecular weight, in g/mol If only DNA length is given, the molecular weight is calculated as: MW = DNA Length (bp) × DNA/RNA base weight The approx. What is the DNA Concentration Calculator? Definition: This calculator computes the concentration of a nucleic acid sample (DNA or RNA) based on its absorbance at 260 nm (A 260), using the Beer-Lambert Law. Use our DNA concentration calculator to convert A260 readings to ng/µL for dsDNA, ssDNA, or RNA, and convert between mass and molar concentration (nM) by fragment length. Mar 25, 2025 · Calculate the mass or volume required to prepare a nucleic acid solution of specified molar concentration. Knowning the DNA copy number is important for designing experiment like Quantitative PCR. Calculate DNA metrics including nucleotide content, molecular weight, and concentration. Additionally, it gives the GC content, moles and copies of ssDNA you enter which are also useful to determine characteristics of DNA. . If needed, modify the recommended primer concentration. Next, select the source of This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for proteins, and vice versa. Do I use beer-lamberts law or calculate concentration from ng/ul concentration using molecular weight of the oligos? This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μl If you know that the weight of your DNA (molar mass per bp) is different from the average weight of DNA basepair, change the value. Calculator start by with standard molar mass per base pair (bp) of your DNA (650 g/mol per base pair). Feb 27, 2023 · Use IDT’s free resuspension and dilution calculators to prepare primers and oligos with precision. 3 days ago · Use VectorBuilder’s DNA calculator to retrieve molecular weight and amount of your DNA (mass or moles) for your experiment like PCR, vector cloning, and more. Convert DNA concentration (ng/µL, µg/mL) to molarity (mol/L, µM, nM) and molecules/µL. DNA Concentration Calculator DNA Concentration Calculator - Measure Nucleic Acid Quantities Precisely Calculate DNA concentration, purity, and molarity from spectrophotometer readings. DNA copy number calculator determines the number of copies of single or double-stranded DNA and RNA in a solution and per PCR cycle. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. Used to determine how […] 1. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Library Concentration Conversion Calculator Consider the xGen™ Normalase™ Module and xGen Normalase indexing primers for enzymatic normalization of up to 1,536 libraries. NEBuilder HiFi DNA assembly efficiently assembles two to six fragments of double-stranded DNA in a single reaction. Keep total DNA concentration between 1-10 µg/ml. How do I calculate DNA concentration in molar using nanodrop? I use a thermo 2000c nanodrop for measuring DNA oligos concentration after PAGE purification. Includes dilution, purity ratios (260/280, 260/230), and step-by-step explanations. List of DNA and RNA Molecular Weights and Conversions including nucleotide molecular weight and rna molecular weight. This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction. The desired molarity ratios between A: B are 5:1, 1:1 and 1:3. The computed parameters include the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficient, estimated half-life, instability index, aliphatic index The Mass Molarity Calculator is commonly used in molecular biology applications that involve calculating a solution's molarity based on the solute's mass and the solvent's volume. Enter primer sequences (with up to 3 ambiguous bases). Whether you start with an OD₂₆₀ reading, a mass concentration, or a molar value, this tool quickly calculates the equivalents. MedChemExpress provides high-quality research chemicals and biochemicals, including inhibitors, proteins, and compound libraries for scientific research and drug discovery. NEBioCalculator can help convert DNA mass concentration to moles. Can anyone tell me the formula to achieve required ratios Nov 15, 2025 · Free DNA ligation calculator for molecular cloning. An important parameter of any DNA assembly reaction is the number of DNA molecules present in the assembly. I have two plasmids: plasmid A (8434bp)and plasmid B (12,082bp). DNA molar mass is the mass of one mole of a DNA molecule, calculated as the sum of the molar masses of its constituent nucleotides. Calculate DNA concentration instantly using absorbance (OD260).

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